C20:4 (arachidonic acid)

metabolite

C20:4 (arachidonic acid) is the precursor for thromboxane-A2 and inflammatory mediator synthesis amplifying thrombotic and inflammatory responses in MI.

Pathway placement

Cascade stepThromboxane amplification

Confidencehigh

RationaleArachidonic acid substrate for COX-1/thromboxane and inflammatory eicosanoid production.

Also acts inVascular inflammation

Druggability

Not assessed (no mapped human gene target).

Assay & specimen

Class-level default (no specific cleared assay)— generic method inferred from analyte class; confirm against a specific product insert before use.

Specimen

Serum, plasma or urine

Collection tube

Serum separator (gold/red-top, SST) · Lithium heparin (green-top) · Sterile urine container

Method / principle

LC-MS/MS (targeted metabolomics) or enzymatic colorimetric where available

Reagent / substrate

Stable-isotope-labeled internal standard (MS); or enzyme-coupled Trinder reagent

Platform

LC-MS/MS; some automated chemistry

Turnaround · availability

Send-out / research · Specialized / research

Literature evidence(1)

Pilot study of myocardial ischemia-induced metabolomic changes in emergency department patients undergoing stress testing.
PloS one · 2019 · PMID 30707740 · doi