Platelet-activating factor

lipid

PAF amplifies platelet activation and recruits leukocytes in coronary thrombogenesis.

Pathway placement

Cascade stepPlatelet adhesion & activation

Confidencehigh

RationalePotent platelet agonist and inflammatory lipid mediator driving thrombosis.

Also acts inVascular inflammation

Druggability

Not assessed (no mapped human gene target).

Type I vs Type II discrimination

ScoresShared / rises in both

R — rupture / Type-I

C — confounder / Type-II

A — assay feasibility

E — evidence strength

T1DI (composite)

Specificity differential (R−C)-6.7

Confounder panel (Type-II drivers)

1sepsis / systemic inflammationmag 3

2anemia / acute blood lossn/a

3hypovolemia / dehydrationmag 2

4tachyarrhythmiamag 2

5hypoxemia / respiratory failuremag 2

6hypertensive emergencyn/a

7high-demand / peri-operative stressmag 2

Coverage: 5/7 confounders with evidence

Tier: deep-scored (abstract-extracted) · 16 supporting references. See the discrimination table for all markers.

Class-level default (no specific cleared assay)— generic method inferred from analyte class; confirm against a specific product insert before use.

Specimen

Serum or plasma (EDTA to limit oxidation)

Collection tube

K2/K3-EDTA (lavender-top) · Serum separator (gold/red-top, SST)

Method / principle

LC-MS/MS lipidomics (targeted or shotgun)

Reagent / substrate

Deuterated lipid-class internal standards; MS/MS transitions

Platform

LC-MS/MS

Turnaround · availability

Research · Research-only