tPA/PAI-1 complex
complextPA/PAI-1 complex imbalance (excess PAI-1) suppresses fibrinolysis and promotes thrombus persistence in coronary occlusion.
Pathway placement
Cascade stepCoagulation & thrombus formation
Confidencehigh
RationaleReflects fibrinolytic-anticoagulant balance; elevated PAI-1 indicates impaired thrombus dissolution.
Druggability
Not assessed (no mapped human gene target).
Assay & specimen
Class-level default (no specific cleared assay)— generic method inferred from analyte class; confirm against a specific product insert before use.
Specimen
Plasma (citrate for coagulation complexes)
Collection tube
Sodium citrate 3.2% (light blue-top) · K2/K3-EDTA (lavender-top)
Method / principle
Sandwich ELISA against neo-complex epitope
Reagent / substrate
Antibody pair recognizing the assembled complex
Platform
ELISA plate
Turnaround · availability
Research / send-out · Research/specialized
Literature evidence(1)
- Predictive value of fibrinolytic factors in coronary heart disease.Scandinavian journal of clinical and laboratory investigation. Supplementum · 1999 · PMID 10389198