Acyl-CoA Synthetase Long Chain Family Member 4
ACSL4proteinAcyl-CoA synthetase that activates polyunsaturated fatty acids for ferroptotic lipid peroxidation, a mechanism linking macrophage oxidative stress to plaque inflammation and instability.
Pathway placement
Cascade stepPlaque inflammation
Confidencehigh
RationaleACSL4 mediates ferroptosis via lipid peroxidation; controls plaque inflammatory cell death and oxidative stress.
Also acts inLipid entry/oxidation
Druggability
DruggableYes
Known drugs / candidates0
Small-molecule tractableNo
Antibody tractableYes
EnsemblENSG00000068366
Type I vs Type II discrimination
ScoresLow-confidence (proxy)
R — rupture / Type-I20
C — confounder / Type-II79
A — assay feasibility68
E — evidence strength29
T1DI (composite)4
Specificity differential (R−C)-59.2
Confounder panel (Type-II drivers)
No confounder evidence retrieved.
Tier: light (literature co-occurrence proxy — lower confidence). See the discrimination table for all markers.
Assay & specimen
Class-level default (no specific cleared assay)— generic method inferred from analyte class; confirm against a specific product insert before use.
Specimen
Serum or plasma
Collection tube
Serum separator (gold/red-top, SST) · K2/K3-EDTA (lavender-top)
Method / principle
Sandwich immunoassay (ELISA) — research-grade unless a cleared assay exists
Reagent / substrate
Matched anti-target antibody pair (capture + labeled detection)
Platform
ELISA microplate or multiplex (Luminex/MSD)
Turnaround · availability
Send-out / research · Research-grade (no universal clinical assay)
Literature evidence(3)
- Xanthosine alleviates myocardial ischemia-reperfusion injury through attenuation of cardiomyocyte ferroptosis.Cellular & molecular biology letters · 2025 · PMID 40722060 · doi
- Comprehensive metagenomic and lipidomic analysis showed that baicalin could improve depressive behaviour in atherosclerotic mice by inhibiting nerve cell ferroptosis.Frontiers in immunology · 2025 · PMID 40977701 · doi
- Integrating metagenomics, lipidomics and proteomics to explore the effect and mechanism of ginsenoside Rb1 on atherosclerosis co-depression disease.Phytomedicine : international journal of phytotherapy and phytopharmacology · 2025 · PMID 41014671 · doi