citrullinated histone H3
H3C1proteinNETosis product serving as specific marker of neutrophil extracellular trap burden and pathogenic plaque inflammation.
Pathway placement
Cascade stepPlaque inflammation
Confidencehigh
RationaleCanonical NET marker produced by PAD4-mediated histone modification.
Also acts inPlatelet activation
Druggability
DruggableNo
Known drugs / candidates0
Small-molecule tractableNo
Antibody tractableNo
EnsemblENSG00000275714
Type I vs Type II discrimination
ScoresLow-confidence (proxy)
R — rupture / Type-I71
C — confounder / Type-II63
A — assay feasibility68
E — evidence strength28
T1DI (composite)10
Specificity differential (R−C)+7.5
Confounder panel (Type-II drivers)
No confounder evidence retrieved.
Tier: light (literature co-occurrence proxy — lower confidence). See the discrimination table for all markers.
Assay & specimen
Class-level default (no specific cleared assay)— generic method inferred from analyte class; confirm against a specific product insert before use.
Specimen
Serum or plasma
Collection tube
Serum separator (gold/red-top, SST) · K2/K3-EDTA (lavender-top)
Method / principle
Sandwich immunoassay (ELISA) — research-grade unless a cleared assay exists
Reagent / substrate
Matched anti-target antibody pair (capture + labeled detection)
Platform
ELISA microplate or multiplex (Luminex/MSD)
Turnaround · availability
Send-out / research · Research-grade (no universal clinical assay)
Literature evidence(2)
- Prothrombotic fibrin clot phenotype as a risk factor for persistent left ventricular thrombus following acute myocardial infarction.Journal of thrombosis and haemostasis : JTH · 2025 · PMID 40505869 · doi
- Linking Neutrophil Extracellular Traps and Platelet Activation: A Composite Biomarker Score for Predicting Outcomes after Acute Myocardial Infarction.Thrombosis and haemostasis · 2021 · PMID 33984869 · doi