Pathway / Myocardial injury (shared endpoint)

NADPH oxidase 4

NOX4gene

NOX4-driven NADPH oxidase activity amplifies oxidative stress and myocardial injury during ischemia-reperfusion.

Pathway placement

Cascade stepMyocardial injury (shared endpoint)

Confidencehigh

RationaleNADPH oxidase mediates oxidative stress and ROS generation in ischemia-reperfusion injury.

Also acts inVascular inflammation

Druggability

DruggableYes

Known drugs / candidates1

Small-molecule tractableYes

Antibody tractableYes

EnsemblENSG00000086991

Type I vs non-Type I discrimination

ScoresNon-Type-I-associated

R — rupture / Type-I

—

C — non-Type-I

A — assay feasibility

E — evidence strength

T1DI (composite)

Specificity differential (R−C)-46.9

Non-Type-I axis panel

Does this marker also move in each non-Type-I setting? mag 0–3; higher means less Type-I-specific. n/a = no evidence retrieved, which is not the same as no change.

2sepsis / systemic inflammationmag 2

2anemia / acute blood lossn/a

2hypovolemia / dehydrationn/a

2tachyarrhythmiamag 2

2hypoxemia / respiratory failuremag 2

2hypertensive emergencymag 2

2high-demand / peri-operative stressmag 1

3sudden cardiac deathmag 2

4aPCI-related periproceduraln/a

4bstent thrombosisn/a

4cin-stent restenosismag 2

5CABG-relatedn/a

Coverage: 7/12 axes with evidence

Tier: deep-scored (abstract-extracted) · 15 supporting references. See the discrimination table for all markers.

Assay & specimen

Class-level default (no specific cleared assay)— generic method inferred from analyte class; confirm against a specific product insert before use.

Specimen

Whole blood — gene is not a circulating analyte; measure protein product or genotype

Collection tube

K2-EDTA whole blood (lavender-top)

Method / principle

SNP genotyping / sequencing; or immunoassay of encoded protein

Reagent / substrate

Allele-specific primers/probes (TaqMan) or NGS panel; or antibody for protein

Platform

qPCR / NGS / array

Turnaround · availability

Send-out · Genotyping widely available; protein assay variable

Literature evidence(1)

Cardiac ischaemia/reperfusion in pigs and mice increases cardiomyocyte Krüppel-like factor 5 that aggravates tissue injury and remodelling.
Cardiovascular research · 2025 · PMID 40079359 · doi