Secreted Frizzled-related protein 3
SFRP3proteinsFRP3 antagonizes canonical Wnt signaling, suppressing endothelial-to-mesenchymal transition and maintaining endothelial barrier function during vascular injury.
Pathway placement
Cascade stepEndothelial activation & erosion
Confidencemedium
RationaleWnt antagonist that inhibits EndMT and preserves endothelial stability.
Also acts inVascular inflammation
Druggability
Not assessed (no mapped human gene target).
Assay & specimen
Class-level default (no specific cleared assay)— generic method inferred from analyte class; confirm against a specific product insert before use.
Specimen
Serum or plasma
Collection tube
Serum separator (gold/red-top, SST) · K2/K3-EDTA (lavender-top)
Method / principle
Sandwich immunoassay (ELISA) — research-grade unless a cleared assay exists
Reagent / substrate
Matched anti-target antibody pair (capture + labeled detection)
Platform
ELISA microplate or multiplex (Luminex/MSD)
Turnaround · availability
Send-out / research · Research-grade (no universal clinical assay)
Literature evidence(1)
- Wnt Site Signaling Inhibitor Secreted Frizzled-Related Protein 3 Protects Mitral Valve Endothelium From Myocardial Infarction-Induced Endothelial-to-Mesenchymal Transition.Journal of the American Heart Association · 2022 · PMID 35348006 · doi