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5,6-EET
Pathway / Off-pathway / systemic markers

5,6-EET

metabolite

5,6-EET is a cytochrome P450-derived eicosanoid associated with lipid-mediated pathophysiology in acute myocardial events.

Pathway placement
Cascade stepOff-pathway / systemic markers
Confidencemedium
RationaleEicosanoid biomarker in AMI; reflects lipid signaling in acute myocardial injury.
Also acts inLipid entry/oxidation
Druggability
Not assessed (no mapped human gene target).

Assay & specimen

Class-level default (no specific cleared assay)— generic method inferred from analyte class; confirm against a specific product insert before use.
Specimen
Serum, plasma or urine
Collection tube
Serum separator (gold/red-top, SST) · Lithium heparin (green-top) · Sterile urine container
Method / principle
LC-MS/MS (targeted metabolomics) or enzymatic colorimetric where available
Reagent / substrate
Stable-isotope-labeled internal standard (MS); or enzyme-coupled Trinder reagent
Platform
LC-MS/MS; some automated chemistry
Turnaround · availability
Send-out / research · Specialized / research

Literature evidence(1)