Caspase
proteinCaspase activation executes apoptosis and cleaves contractile proteins in cardiomyocytes during ischemic injury and reperfusion-mediated cell death.
Pathway placement
Cascade stepMyocardial injury (shared endpoint)
Confidencemedium
RationaleApoptotic protease; cardiomyocyte death in ischemia-reperfusion.
Druggability
Not assessed (no mapped human gene target).
Type I vs Type II discrimination
ScoresShared / rises in both
R — rupture / Type-I67
C — confounder / Type-II76
A — assay feasibility68
E — evidence strength60
T1DI (composite)19
Specificity differential (R−C)-9
Confounder panel (Type-II drivers)
1sepsis / systemic inflammationmag 3
2anemia / acute blood lossmag 2
3hypovolemia / dehydrationmag 2
4tachyarrhythmiamag 3
5hypoxemia / respiratory failuremag 2
6hypertensive emergencymag 2
7high-demand / peri-operative stressmag 2
Coverage: 7/7 confounders with evidence
Tier: deep-scored (abstract-extracted) · 23 supporting references. See the discrimination table for all markers.
Assay & specimen
Class-level default (no specific cleared assay)— generic method inferred from analyte class; confirm against a specific product insert before use.
Specimen
Serum or plasma
Collection tube
Serum separator (gold/red-top, SST) · K2/K3-EDTA (lavender-top)
Method / principle
Sandwich immunoassay (ELISA) — research-grade unless a cleared assay exists
Reagent / substrate
Matched anti-target antibody pair (capture + labeled detection)
Platform
ELISA microplate or multiplex (Luminex/MSD)
Turnaround · availability
Send-out / research · Research-grade (no universal clinical assay)
Literature evidence(1)
- Degradation of troponin I in serum or plasma: mechanisms, and analytical and clinical implications.Seminars in thrombosis and hemostasis · 2012 · PMID 22422336 · doi
Clinical trials(1)
- The Role of Colchicine in Reducing The Rate of Myocardial Reperfusion Injury in Patients With ST-Elevation Myocardial Infarction After Primary Percutaneous Coronary Intervention: Study on NLRP3, ASC, Caspase, and TroponinNCT05734612 · PHASE3 · UNKNOWN · Colchicine, Placebo (lactose)