Pathway / Myocardial injury (shared endpoint)

HtrA2/Omi protease

protein

A mitochondrial serine protease released into circulation during ischemic cardiomyocyte apoptosis, marking myocardial necrosis in acute ST-elevation infarction.

Pathway placement

Cascade stepMyocardial injury (shared endpoint)

Confidencemedium

RationaleMitochondrial protease released during cardiomyocyte apoptosis in STEMI.

Druggability

Not assessed (no mapped human gene target).

Assay & specimen

Class-level default (no specific cleared assay)— generic method inferred from analyte class; confirm against a specific product insert before use.

Specimen

Serum or plasma

Collection tube

Serum separator (gold/red-top, SST) · K2/K3-EDTA (lavender-top)

Method / principle

Sandwich immunoassay (ELISA) — research-grade unless a cleared assay exists

Reagent / substrate

Matched anti-target antibody pair (capture + labeled detection)

Platform

ELISA microplate or multiplex (Luminex/MSD)

Turnaround · availability

Send-out / research · Research-grade (no universal clinical assay)

Literature evidence(1)

Circulating HtrA2 as a novel biomarker for mitochondrial induced cardiomyocyte apoptosis and ischemia-reperfusion injury in ST-segment elevation myocardial infarction.
International journal of cardiology · 2017 · PMID 28602551 · doi