microRNA-32-5p

rna

miR-32-5p impairs endothelial KLF2 expression, compromising endothelial mechanotransduction and promoting erosion-type MI.

Pathway placement

Cascade stepEndothelial activation & erosion

Confidencemedium

RationaleDiagnostic biomarker for AMI; targets KLF2, a regulator of endothelial cell viability and mechanotransduction.

Also acts inVascular inflammation

Druggability

Not assessed (no mapped human gene target).

Assay & specimen

Class-level default (no specific cleared assay)— generic method inferred from analyte class; confirm against a specific product insert before use.

Specimen

Whole blood or cell-free plasma

Collection tube

PAXgene/Tempus RNA tube · K2/K3-EDTA (lavender-top)

Method / principle

RT-qPCR (TaqMan) or small-RNA sequencing

Reagent / substrate

Target-specific primers/probe; reverse transcriptase (miRNA: stem-loop RT primer)

Platform

qPCR instrument / NGS

Turnaround · availability

Research / send-out · Research (few LDTs)