Complement C5b-9 (MAC)

complex

C5b-9 amplifies endothelial injury and inflammatory cell recruitment through complement-driven lysis and activation.

Pathway placement

Cascade stepPlaque inflammation

Confidencehigh

RationaleComplement membrane-attack complex; direct inflammation marker elevated in AMI.

Also acts inEndothelial activation/erosion

Druggability

Not assessed (no mapped human gene target).

Type I vs Type II discrimination

ScoresType-II-associated

R — rupture / Type-I

—

C — confounder / Type-II

A — assay feasibility

E — evidence strength

T1DI (composite)

Specificity differential (R−C)-52

Confounder panel (Type-II drivers)

1sepsis / systemic inflammationmag 2

2anemia / acute blood lossn/a

3hypovolemia / dehydrationn/a

4tachyarrhythmian/a

5hypoxemia / respiratory failuren/a

6hypertensive emergencyn/a

7high-demand / peri-operative stressn/a

Coverage: 1/7 confounders with evidence

Tier: deep-scored (abstract-extracted) · 2 supporting references. See the discrimination table for all markers.

Class-level default (no specific cleared assay)— generic method inferred from analyte class; confirm against a specific product insert before use.

Specimen

Plasma (citrate for coagulation complexes)

Collection tube

Sodium citrate 3.2% (light blue-top) · K2/K3-EDTA (lavender-top)

Method / principle

Sandwich ELISA against neo-complex epitope

Reagent / substrate

Antibody pair recognizing the assembled complex

Platform

ELISA plate

Turnaround · availability

Research / send-out · Research/specialized